Short-term and long-term habituation along with the effects of light intensity on the response to closing the habituation flaps in Mimosa Pudica for habituation researchFuture researchMore recently, Gagliano et al. al., studied short- and long-term habituation along with the effects of light intensity on the habituation flap-closing response in Mimosa pudica. The results of the experiment confirmed the findings of Holmes and Grunberg. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an Original EssayApparatus and EquipmentThe apparatus consists of a vertical steel railing attached to a foam base. A clear acrylic pot is mounted on the steel railing using variable hooks where the potted plants will be placed during the experiment. The foam base is cut and hollowed out in the center creating a shallow depression into which the acrylic vessel can drop from above by sliding it along the steel railing. Training Procedure Set up a growth room with a size of 5.30 m2 for the experiments. Make three compartments in the room using black plastic sheets. The training tests will be conducted in the central compartment while the test tests will be conducted in the lateral compartments. In each of the side compartments there are fluorescent lights, one compartment with low intensity lights (LL) while the other compartment with high intensity lights (HL). Measure the light intensity just above the plants in each compartment before starting the experiment. Grow each individual Mimosa pudica plant to similar heights (6-8cm) in a 10cm round acrylic pot with a standard mix of loamy soil and organic compost at a volume of 1:1. Fertilize and water plants as needed. Maintain a 12-hour light: 12-hour dark cycle, 60%-70% relative humidity, and 70-70°F temperature. Habit training Randomly assign individual potted plants to HL or LL conditions. Leave them undisturbed for 5 days until training day. Place a potted plant in the acrylic vase and drop it from a set height of 15cm in the morning, then repeat the drop from the same height 8 hours later. Repeat the operation for all the plants in each of the side compartments. Place a potted plant in the acrylic vase and drop it from a designated height of 15cm. Repeat this procedure 60 times with an interval of 5 to 10 seconds between each drop. Repeat another series of 60 drops, a total of seven consecutive times one after the other in a single day with increasing intervals between each series of 60 drops. Repeat the operation for all the plants from each of the side compartments. Dishousing Test Place the single plant in a tight-fitting foam container attached to a shaker plate. Turn on the stirrer plate and set it to 250 rpm for 5 seconds. Observe leaf bending behavior. Test for short-term memory of the habitual response to leaf bending. Test each trained plant from both lateral compartments 6 days after 1 day of training using 60 consecutive drops from the set height of 15 cm .Test for long-term memory in Mimosa exposed to a new environment. Transfer plants from the lateral compartment with LL to the compartment with HL and vice versa. Test the plants after 28 days using the complete training protocol mentioned above in training section 2. Data Analysis After each drop, for each individual plant select three random leaves. Measure the.

Topic > Short-term and long-term habituation along with the effects of light intensity on the response to closing the habituation flaps in Mimosa Pudica for habituation researchFuture researchMore recently, Gagliano et al. al., studied short- and long-term habituation along with the effects of light intensity on the habituation flap-closing response in Mimosa pudica. The results of the experiment confirmed the findings of Holmes and Grunberg. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an Original EssayApparatus and EquipmentThe apparatus consists of a vertical steel railing attached to a foam base. A clear acrylic pot is mounted on the steel railing using variable hooks where the potted plants will be placed during the experiment. The foam base is cut and hollowed out in the center creating a shallow depression into which the acrylic vessel can drop from above by sliding it along the steel railing. Training Procedure Set up a growth room with a size of 5.30 m2 for the experiments. Make three compartments in the room using black plastic sheets. The training tests will be conducted in the central compartment while the test tests will be conducted in the lateral compartments. In each of the side compartments there are fluorescent lights, one compartment with low intensity lights (LL) while the other compartment with high intensity lights (HL). Measure the light intensity just above the plants in each compartment before starting the experiment. Grow each individual Mimosa pudica plant to similar heights (6-8cm) in a 10cm round acrylic pot with a standard mix of loamy soil and organic compost at a volume of 1:1. Fertilize and water plants as needed. Maintain a 12-hour light: 12-hour dark cycle, 60%-70% relative humidity, and 70-70°F temperature. Habit training Randomly assign individual potted plants to HL or LL conditions. Leave them undisturbed for 5 days until training day. Place a potted plant in the acrylic vase and drop it from a set height of 15cm in the morning, then repeat the drop from the same height 8 hours later. Repeat the operation for all the plants in each of the side compartments. Place a potted plant in the acrylic vase and drop it from a designated height of 15cm. Repeat this procedure 60 times with an interval of 5 to 10 seconds between each drop. Repeat another series of 60 drops, a total of seven consecutive times one after the other in a single day with increasing intervals between each series of 60 drops. Repeat the operation for all the plants from each of the side compartments. Dishousing Test Place the single plant in a tight-fitting foam container attached to a shaker plate. Turn on the stirrer plate and set it to 250 rpm for 5 seconds. Observe leaf bending behavior. Test for short-term memory of the habitual response to leaf bending. Test each trained plant from both lateral compartments 6 days after 1 day of training using 60 consecutive drops from the set height of 15 cm .Test for long-term memory in Mimosa exposed to a new environment. Transfer plants from the lateral compartment with LL to the compartment with HL and vice versa. Test the plants after 28 days using the complete training protocol mentioned above in training section 2. Data Analysis After each drop, for each individual plant select three random leaves. Measure the.