Demineralization (DM), deproteinization (DP), decolorization and deacetiation are the basic steps in chitosan production; this process is expensive, thus limiting wider applications of chitosan. Simplifying chitosan production by eliminating the DM or DP step or reducing the reaction time required for DM and DP (No et al., 2003) would considerably reduce production costs by reducing the use of chemicals , process times and bulky wastewater discharge. However, chitosan can be prepared by a simple method for some uses, such as coating eggs. Because this does not require pure chitosan (No et al., 2005). There are two types of chitosan such as α-chitosan and β-chitosan based on the origin (Kim et al., 2007). Chitosan is not dissolved in water or organic solvents, but is soluble in dilute organic acids such as acetic acid, lactic acid, and forms viscous solutions (Kim et al., 2006; Chen and Zhao, 2012). Sealing characteristics can vary based on the molecular weight of the chitosan (Bhale et al., 2003) and the type of solvent (Kim et al..,
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