Index1.0 Introduction 31.1 Objective 31.2 Fundamentals 31.3 Analysis of the technique 42.0 Experimental procedure 63.0 Results 83.1 Vanillin concentration table with absorbance 83.2 Calculation 94.0 Discussion 105.0 Conclusion 116.0 Reference 121.0 Introduction1.1 ObjectiveThe objective of this experiment is to analyze the concentration of the active ingredient vanillin in artificial vanilla essence using UV-visible spectrometry. 1.2 Background Vanilla is one of the most common flavors used in addictive foods, such as ice cream and milk. There are two types of vanilla: pure vanilla and imitation vanilla. Pure vanilla is made from the vanilla pod while imitation vanilla is made from clove oil which is extracted from the pod then combined with ethanol and sugar or glycerin (corresponding, 2003). Inside vanilla is a molecule called vanillin or known as 4-hydroxy-3-methoxybenzaldehyde. The chemical bond structure of vanillin is shown in figure 1 below. The vanillin contained in vanilla mainly serves to impart the flavor and smell of vanilla. Today, the consumption of pure vanilla is lower than that of imitation vanilla because its production requires time and labor. Therefore the cost of vanilla extract is higher than that of imitation vanilla (corresponding, 2003). Figure 1: Vanillin (Wikimedia Common, 2014) 1.3 Technical analysis The determination of the vanilla concentration in this experiment involves three main steps, which are the extraction of vanillin from the other components of the essence using an organic solvent, back extraction of vanillin as its sodium salt in the aqueous phase using dilute sodium hydroxide and determination of vanillin concentration... in the center of the paper... with greater data accuracy.6.0 ReferenceCorrespondent, DY (2003). Daytona Beach News - Newspaper, The. Knowing Beans on Vanilla, Imitation: [WEST VOLUSIA Edition].Elder, K. (n.d.). How accurate are your experiments? Retrieved from Kennewick High School Science: http://kehsscience.org/How%20accurate%20are%20your%20experiments.pdfOliva-Chatelain, B.L., & Barron, AR (2010). Basics of UV-visible spectroscopy. Retrieved from openstax cnx: http://cnx.org/content/m34525/latest/Schneider, R. F. (2013). Retrieved from Beers Law: http://www.ic.sunysb.edu/Class/che133/lectures/beerslaw.htmlW. H. Bunnelle, L.A. (n.d.). Extraction. Retrieved from University of Missouri: http://www.chem.missouri.edu/chem2050/expt_2.pdfWikimedia Common. (2014). File: Vanillina.png. Retrieved from Wikimedia Common: http://commons.wikimedia.org/wiki/File:Vanillin.png